Skin lightening and tanning are two major areas dominating the market of skincare\nand cosmetic products. Though the demands are originated from two different\ncommunities, the two areas share a same goalââ?¬â?skin colour tuning. The known safe\ncompounds with skin colour tuning activities are limited. In contrary, Chinese medicinal\nherb provides a pool of natural bioactive compounds, which have been used in\nAsian countries for long time and have been tested for its toxicity. Here, we demonstrate\na high throughput screening platform for potential compounds usable for skin\ncolour tuning. From 147 natural compounds, 26 of them showed potential in skin\ntanning functions by using the high throughput melanogenesis platform based on\nthe melanogenesis assay on B16 melanocytes. Five of them promoted melanogenesis\nby over 50%. Moreover, apart from 1, 8-dihydroxyanthraquinone, the other four\ncompounds showed enhancement effect on tyrosinase activity. From the result, the\ncompounds increased the Vmax of tyrosinase without changing the Km in a dosedependent\nmanner. Thus, there should be no irreversible structural change of the\nenzyme. Definitely, this report contributes to the development of personalization in\nskincare and cosmetic products.
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